Potential of Millet Grains as Alternative Media for Preservation of Fungal Pathogen
نویسندگان
چکیده
Abstract Crop losses due to pathogen attacks had increased tremendously from year and this situation is very alarming. Among important pathogen, fungi are dominant causal agents of plant disease that manage colonize the host with various strategies finally kill them. Identification based on laboratory investigations become vital since there many kinds linked disease. Laboratory might take a long period thus effective storage preservation fungal cultures critical ensuring their viability maintaining genetic integrity. Conventional agar medium not really ideal for long-term study it requires repetitive sub-culturing, which can change pathogen’s characteristics contaminate cultures. This has determined potential millet grains as alternative media using three different drying process. Two species related dieback woody plants such Parkinsonia, mango, cocoa apple have been used in namely Lasiodiplodia pseudotheobromae Neoscytalidium novaehollandiae . The effectiveness culture were assessed contamination rates. Millet cultured dried process air drying, oven freeze drying. Viability test revealed both produced high rates even after 8 weeks grains. least rate recorded by findings indicate habe great an pathogen.
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ژورنال
عنوان ژورنال: IOP conference series
سال: 2022
ISSN: ['1757-899X', '1757-8981']
DOI: https://doi.org/10.1088/1755-1315/1059/1/012017